DnaK (AA 508-638) Protéine
Aperçu rapide pour DnaK (AA 508-638) Protéine (ABIN666807)
Antigène
Type de proteíne
Origine
Source
Application
Pureté
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Attributs du protein
- AA 508-638
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Séquence
- MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIE SALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADA SANNAKDDDV VDAEFEEVKD KK
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Attributs du produit
- Dnak(508-638), E.coli, Recombinant, E.coli
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Purification
- > 95% by SDS-PAGE
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 25 mM Tris-HCl buffer ( pH 7.5) containing 100 mM NaCl, 10 % glycerol
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Stock
- 4 °C,-20 °C,-80 °C
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Stockage commentaire
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- DnaK
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Sujet
- DnaK, originally identified for its DNA replication by bacteriophage lambda in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak (residues 508-638) of the substrate binding domain is alpha-helical and appears to act as a lid covering the substrate binding cleft. DnaK (amino acid 508-638) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus
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Poids moléculaire
- 14.6 kDa (132aa)
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NCBI Accession
- NP_414555
Antigène
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